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KMID : 0616120210520020105
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Korean Journal of Pharmacognosy
2021 Volume.52 No. 2 p.105 ~ p.111
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Antioxidant Activities of n-Butanol Fraction of Pinus densiflora Siebold & Zucc. Needle in Caenorhabditis elegans
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Kim Jun-Hyeong
Kim Na-Yeong
Kwon Kang-Mu
Hwang In-Hyun
Kim Dae-Keun
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Abstract
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To investigate the antioxidant activities of Pinus densiflora needle (Pinaceae), which is widely used as a traditional medicine in Korea, Caenorhabditis elegans model system was used. The n-butanol fraction of P. densiflora needle methanol extract showed the most potent DPPH radical scavenging and superoxide quenching activities in a dose-dependent manner. The n-butanol fraction was measured for the activities of superoxide dismutase (SOD), catalase, and oxidative stress tolerance by using C. elegans with reactive oxygen species (ROS) level. In addition, to see that the regulation of the stress response gene is responsible for the increased stress tolerance of C. elegans treated by the butanol extract, SOD-3 expression was measured using a transgenic strain. As a result, the P. densiflora needle n-butanol fraction increased SOD and catalase activities, and decreased ROS accumulation, dose-dependently. Furthermore, the n-butanol fraction-treated CF1553 worm showed higher SOD-3::GFP intensity than the control.
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KEYWORD
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Pinus densiflora, Caenorhabditis elegans, Antioxidant, SOD, Catalase, ROS
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